2JH3: The Crystal Structure Of Dr2241 From Deinococcus Radiodurans At 1.9 A Resolution Reveals A Multi-Domain Protein With Structural Similarity To Chelatases But Also With Two Additional Novel Domains

A unique family of proteins have been identified in the Deinococcus genus with an N-terminal cobalamin (vitamin B(12)) chelatase domain denoted CbiX and an additional unique C-terminal domain with unknown function. Here we report the first crystal structure from this new family of proteins with the structure of Deinococcus radiodurans protein DR2241. The structure reveals a multi-domain protein where domains A (residues 1-132) has the same fold as the small CbiX (CbiX(S)), domains A and B (residues 1-272) follow the chelatase super-family fold and the two additional unique domains C and D have no structural homologues. Domain D harbours the sequence motifs CxxC and CxxxC, in which DR2241 gives the first evidence that these motifs bind a [4Fe-4S] iron-sulphur cluster. In solution there are indications of multimeric forms, and in the crystallographic asymmetric unit a tetramer is found where domains C and D are involved in stabilising the tetrameric assembly.
PDB ID: 2JH3Download
MMDB ID: 46921
PDB Deposition Date: 2007/2/20
Updated in MMDB: 2007/10
Experimental Method:
x-ray diffraction
Resolution: 1.9  Å
Source Organism:
Similar Structures:
Biological Unit for 2JH3: dimeric; determined by author and by software (PQS)
Molecular Components in 2JH3
Label Count Molecule
Proteins (2 molecules)
Ribosomal Protein S2-related Protein
Molecule annotation
Chemicals (2 molecules)
* Click molecule labels to explore molecular sequence information.

Citing MMDB