2JAK: Human Pp2a Regulatory Subunit B56g

The PP2A serine/threonine phosphatase regulates a plethora of cellular processes. In the cell the predominant form of the enzyme is a heterotrimer, formed by a core dimer composed of a catalytic and a scaffolding subunit, which assemble together with one of a range of different regulatory B subunits. Here, we present the first structure of a free non-complexed B subunit, B56 gamma. Comparison with the recent structures of a heterotrimeric complex and the core dimer reveals several significant conformational changes in the interface region between the B56 gamma and the core dimer. These allow for an assembly scheme of the PP2A holoenzyme to be put forth where B56 gamma first complexes with the scaffolding subunit and subsequently binds to the catalytic subunit and this induces the formation of a binding site for the invariant C-terminus of the catalytic subunit that locks in the complex as a last step of assembly.
PDB ID: 2JAKDownload
MMDB ID: 43476
PDB Deposition Date: 2006/11/29
Updated in MMDB: 2012/12
Experimental Method:
x-ray diffraction
Resolution: 2.6  Å
Source Organism:
Similar Structures:
Biological Unit for 2JAK: monomeric; determined by author and by software (PQS)
Molecular Components in 2JAK
Label Count Molecule
Protein (1 molecule)
Serine/threonine-protein Phosphatase 2A 56 KDA Regulatory Subunit Gamma Isoform(Gene symbol: PPP2R5C)
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB