2J6Z: Structural and functional characterisation of partner-switching regulating the environmental stress response in B. subtilis

The general stress response of Bacillus subtilis and close relatives provides the cell with protection from a variety of stresses. The upstream component of the environmental stress signal transduction cascade is activated by the RsbT kinase that switches binding partners from a 25 S macromolecular complex, the stressosome, to the RsbU phosphatase. Once the RsbU phosphatase is activated by interacting with RsbT, the alternative sigma factor, sigmaB, directs transcription of the general stress regulon. Previously, we demonstrated that the N-terminal domain of RsbU mediates the binding of RsbT. We now describe residues in N-RsbU that are crucial to this interaction by experimentation both in vitro and in vivo. Furthermore, crystal structures of the N-RsbU mutants provide a molecular explanation for the loss of interaction. Finally, we also characterize mutants in RsbT that affect binding to both RsbU and a simplified, binary model of the stressosome and thus identify overlapping binding surfaces on the RsbT "switch."
PDB ID: 2J6ZDownload
MMDB ID: 74723
PDB Deposition Date: 2006/10/5
Updated in MMDB: 2009/07
Experimental Method:
x-ray diffraction
Resolution: 1.95  Å
Source Organism:
Similar Structures:
Biological Unit for 2J6Z: dimeric; determined by author and by software (PQS)
Molecular Components in 2J6Z
Label Count Molecule
Proteins (2 molecules)
Phosphoserine Phosphatase Rsbu(Gene symbol: rsbU)
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB