2J10: p53 tetramerization domain mutant T329F Q331K

Citation:
Abstract
The role of hydrophobic amino acids in the formation of hydrophobic cores as one of the major driving forces in protein folding has been extensively studied. However, the implication of neutral solvent-exposed amino acids is less clear and available information is scarce. We have used a combinatorial approach to study the structural relevance of three solvent-exposed residues (Tyr(327), Thr(329), and Gln(331)) located in thebeta-sheet of the tetramerization domain of the tumor suppressor p53 (p53TD). A conformationally defined peptide library was designed where these three positions were randomized. The library was screened for tetramer stability. A set of p53TD mutants containing putative stabilizing or destabilizing residue combinations was synthesized for a thermodynamic characterization. Unfolding experiments showed a wide range of stabilities, with T(m) values between 27 and 83 degrees C. Wild type p53TD and some highly destabilized and stabilized mutants were further characterized. Thermodynamic and biophysical data indicated that these proteins were folded tetramers, with the same overall structure, in equilibrium with unfolded monomers. An NMR study confirmed that the main structural features of p53TD are conserved in all the mutants analyzed. The thermodynamic stability of the different p53TD mutants showed a strong correlation with parameters that favor formation and stabilization of the beta-sheet. We propose that stabilization through hydrophobic interactions of key secondary structure elements might be the underlying mechanism for the strong influence of solvent-exposed residues in the stability of p53TD.
PDB ID: 2J10Download
MMDB ID: 59031
PDB Deposition Date: 2006/8/8
Updated in MMDB: 2007/12
Experimental Method:
solution nmr
Similar Structures:
Biological Unit for 2J10: tetrameric; determined by author
Molecular Components in 2J10
Label Count Molecule
Proteins (4 molecules)
4
Cellular Tumor Antigen P53(Gene symbol: TP53)
Molecule annotation
* Click molecule labels to explore molecular sequence information.

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