2IY1: Senp1 (mutant) Full Length Sumo1

Small ubiquitin-like modifier (SUMO)-specific protease SENP1 processes SUMO-1, SUMO-2 and SUMO-3 to mature forms and deconjugates them from modified proteins. To establish the proteolytic mechanism, we determined structures of catalytically inactive SENP1 bound to SUMO-1-modified RanGAP1 and to unprocessed SUMO-1. In each case, the scissile peptide bond is kinked at a right angle to the C-terminal tail of SUMO-1 and has the cis configuration of the amide nitrogens. SENP1 preferentially processes SUMO-1 over SUMO-2, but binding thermodynamics of full-length SUMO-1 and SUMO-2 to SENP1 and K(m) values for processing are very similar. However, k(cat) values differ by 50-fold. Thus, discrimination between unprocessed SUMO-1 and SUMO-2 by SENP1 is based on a catalytic step rather than substrate binding and is likely to reflect differences in the ability of SENP1 to correctly orientate the scissile bonds in SUMO-1 and SUMO-2.
PDB ID: 2IY1Download
MMDB ID: 41252
PDB Deposition Date: 2006/7/11
Updated in MMDB: 2007/10
Experimental Method:
x-ray diffraction
Resolution: 2.46  Å
Source Organism:
Similar Structures:
Biological Unit for 2IY1: dimeric; determined by author and by software (PQS)
Molecular Components in 2IY1
Label Count Molecule
Proteins (2 molecules)
Sentrin-specific Protease 1(Gene symbol: SENP1)
Molecule annotation
Small Ubiquitin-related Modifier 1(Gene symbol: SUMO1)
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB