2IWQ: 7th Pdz Domain Of Multiple Pdz Domain Protein Mpdz

Citation:
Abstract
PDZ domains are protein-protein interaction modules that generally bind to the C termini of their target proteins. The C-terminal four amino acids of a prospective binding partner of a PDZ domain are typically the determinants of binding specificity. In an effort to determine the structures of a number of PDZ domains we have included appropriate four residue extensions on the C termini of PDZ domain truncation mutants, designed for self-binding. Multiple truncations of each PDZ domain were generated. The four residue extensions, which represent known specificity sequences of the target PDZ domains and cover both class I and II motifs, form intermolecular contacts in the expected manner for the interactions of PDZ domains with protein C termini for both classes. We present the structures of eight unique PDZ domains crystallized using this approach and focus on four which provide information on selectivity (PICK1 and the third PDZ domain of DLG2), binding site flexibility (the third PDZ domain of MPDZ), and peptide-domain interactions (MPDZ 12th PDZ domain). Analysis of our results shows a clear improvement in the chances of obtaining PDZ domain crystals by using this approach compared to similar truncations of the PDZ domains without the C-terminal four residue extensions.
PDB ID: 2IWQDownload
MMDB ID: 62170
PDB Deposition Date: 2006/7/3
Updated in MMDB: 2012/11
Experimental Method:
x-ray diffraction
Resolution: 1.8  Å
Source Organism:
Similar Structures:
Biological Unit for 2IWQ: dimeric; determined by software (PISA)
Molecular Components in 2IWQ
Label Count Molecule
Proteins (2 molecules)
2
Multiple PDZ Domain Protein(Gene symbol: MPDZ)
Molecule annotation
* Click molecule labels to explore molecular sequence information.

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