2ISO: Ternary Complex Of Dna Polymerase Beta With A Dideoxy Terminated Primer And 2'-Deoxyguanosine 5'-Beta, Gamma- Difluoromethylene Triphosphate

Citation:
Abstract
DNA polymerase catalysis and fidelity studies typically compare incorporation of "right" versus "wrong" nucleotide bases where the leaving group is pyrophosphate. Here we use dGTP analogues replacing the beta,gamma-bridging O with CH2, CHF, CF2, or CCl2 to explore leaving-group effects on the nucleotidyl transfer mechanism and fidelity of DNA polymerase (pol) beta. T.G mismatches occur with fidelities similar to dGTP with the exception of the CH2 analogue, which is incorporated with 5-fold higher fidelity. All analogues are observed to bind opposite template C with Kds between 1 and 4 microM, and structural evidence suggests that the analogues bind in essentially the native conformation, making them suitable substrates for probing linear free energy relationships (LFERs) in transient-kinetics experiments. Importantly, Brnsted correlations of log(kpol) versus leaving-group pKa for both right and wrong base incorporation reveal similar sensitivities (betalg approximately -0.8) followed by departures from linearity, suggesting that a chemical step rather than enzyme conformational change is rate-limiting for either process. The location of the breaks relative to pKas of CF2, O, and the sterically bulky CCl2-bridging compounds suggests a modification-induced change in the mechanism by stabilization of leaving-group elimination. The results are addressed theoretically in terms of the energetics of successive primer 3'-O addition (bond forming) and pyrophosphate analogue elimination (bond breaking) reaction energy barriers.
PDB ID: 2ISODownload
MMDB ID: 105362
PDB Deposition Date: 2006/10/18
Updated in MMDB: 2012/11
Experimental Method:
x-ray diffraction
Resolution: 2.1  Å
Source Organism:
synthetic construct
Similar Structures:
Biological Unit for 2ISO: tetrameric; determined by author
Molecular Components in 2ISO
Label Count Molecule
Protein (1 molecule)
1
Polymerase (Dna Directed), Beta
Molecule annotation
Nucleotides(3 molecules)
1
5'- D(*cp*cp*gp*ap*cp*cp*gp*cp*gp*cp*ap*tp*cp*ap*gp*c)-3'
Molecule annotation
1
5'-d(*gp*cp*tp*gp*ap*tp*gp*cp*gp*(doc))-3'
Molecule annotation
1
5'-d(p*gp*tp*cp*gp*g)-3'
Molecule annotation
Chemicals (9 molecules)
1
1
2
3
3
4
4
1
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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