2HIT: Reaction centre from Rhodobacter sphaeroides strain R-26.1 complexed with dibrominated phosphatidylethanolamine

Citation:
Abstract
This study describes the use of brominated phospholipids to distinguish between lipid and detergent binding sites on the surface of a typical alpha-helical membrane protein. Reaction centers isolated from Rhodobacter sphaeroides were cocrystallized with added brominated phospholipids. X-ray structural analysis of these crystals has revealed the presence of two lipid binding sites from the characteristic strong X-ray scattering from the bromine atoms. These results demonstrate the usefulness of this approach to mapping lipid binding sites at the surface of membrane proteins.
PDB ID: 2HITDownload
MMDB ID: 45016
PDB Deposition Date: 2006/6/29
Updated in MMDB: 2007/10
Experimental Method:
x-ray diffraction
Resolution: 2.75  Å
Source Organism:
Similar Structures:
Biological Unit for 2HIT: trimeric; determined by author and by software (PISA)
Molecular Components in 2HIT
Label Count Molecule
Proteins (3 molecules)
1
Reaction Center Protein L Chain
Molecule annotation
1
Reaction Center Protein M Chain
Molecule annotation
1
Reaction Center Protein H Chain
Molecule annotation
Chemicals (27 molecules)
1
4
2
2
3
2
4
2
5
1
6
3
7
3
8
1
9
1
10
6
11
1
12
1
* Click molecule labels to explore molecular sequence information.

Citing MMDB
.