2GV5: Crystal Structure Of Sfi1p/cdc31p Complex

Citation:
Abstract
Centrins are calmodulin-like proteins present in centrosomes and yeast spindle pole bodies (SPBs) and have essential functions in their duplication. The Saccharomyces cerevisiae centrin, Cdc31p, binds Sfi1p on multiple conserved repeats; both proteins localize to the SPB half-bridge, where the new SPB is assembled. The crystal structures of Sfi1p-centrin complexes containing several repeats show Sfi1p as an alpha helix with centrins wrapped around each repeat and similar centrin-centrin contacts between each repeat. Electron microscopy (EM) shadowing of an Sfi1p-centrin complex with 15 Sfi1 repeats and 15 centrins bound showed filaments 60 nm long, compatible with all the Sfi1 repeats as a continuous alpha helix. Immuno-EM localization of the Sfi1p N and C termini showed Sfi1p-centrin filaments spanning the length of the half-bridge with the Sfi1p N terminus at the SPB. This suggests a model for SPB duplication where the half-bridge doubles in length by association of the Sfi1p C termini, thereby providing a new Sfi1p N terminus to initiate SPB assembly.
PDB ID: 2GV5Download
MMDB ID: 40035
PDB Deposition Date: 2006/5/2
Updated in MMDB: 2012/11
Experimental Method:
x-ray diffraction
Resolution: 3  Å
Source Organism:
Similar Structures:
Biological Unit for 2GV5: trimeric; determined by author and by software (PISA)
Molecular Components in 2GV5
Label Count Molecule
Proteins (3 molecules)
2
Cell Division Control Protein 31(Gene symbol: CDC31)
Molecule annotation
1
Sfi1p(Gene symbol: SFI1)
Molecule annotation
* Click molecule labels to explore molecular sequence information.

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