2GM5: An Activated, Truncated Gamma-delta Resolvase Tetramer

The structures of two mutants of the site-specific recombinase, gammadelta resolvase, that form activated tetramers have been determined. One, at 3.5-A resolution, forms a synaptic intermediate of resolvase that is covalently linked to two cleaved DNAs, whereas the other is of an unliganded structure determined at 2.1-A resolution. Comparisons of the four known tetrameric resolvase structures show that the subunits interact through the formation of a common core of four helices. The N-terminal halves of these helices superimpose well on each other, whereas the orientations of their C termini are more variable. The catalytic domains of resolvase in the unliganded structure are arranged asymmetrically, demonstrating that their positions can move substantially while preserving the four-helix core that forms the tetramer. These results suggest that the precleavage synaptic tetramer of gammadelta resolvase, whose structure is not known, may be formed by a similar four-helix core, but differ in the relative orientations of its catalytic and DNA-binding domains.
PDB ID: 2GM5Download
MMDB ID: 40004
PDB Deposition Date: 2006/4/5
Updated in MMDB: 2017/10
Experimental Method:
x-ray diffraction
Resolution: 2.1  Å
Source Organism:
Similar Structures:
Biological Unit for 2GM5: tetrameric; determined by author and by software (PISA)
Molecular Components in 2GM5
Label Count Molecule
Proteins (4 molecules)
Transposon Gamma-delta Resolvase(Gene symbol: tnpR)
Molecule annotation
* Click molecule labels to explore molecular sequence information.

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