2G9N: Structure Of The Dead Domain Of Human Eukaryotic Initiation Factor 4a, Eif4a

DEAD-box RNA helicases play various, often critical, roles in all processes where RNAs are involved. Members of this family of proteins are linked to human disease, including cancer and viral infections. DEAD-box proteins contain two conserved domains that both contribute to RNA and ATP binding. Despite recent advances the molecular details of how these enzymes convert chemical energy into RNA remodeling is unknown. We present crystal structures of the isolated DEAD-domains of human DDX2A/eIF4A1, DDX2B/eIF4A2, DDX5, DDX10/DBP4, DDX18/myc-regulated DEAD-box protein, DDX20, DDX47, DDX52/ROK1, and DDX53/CAGE, and of the helicase domains of DDX25 and DDX41. Together with prior knowledge this enables a family-wide comparative structural analysis. We propose a general mechanism for opening of the RNA binding site. This analysis also provides insights into the diversity of DExD/H- proteins, with implications for understanding the functions of individual family members.
PDB ID: 2G9NDownload
MMDB ID: 38425
PDB Deposition Date: 2006/3/7
Updated in MMDB: 2010/10
Experimental Method:
x-ray diffraction
Resolution: 2.25  Å
Source Organism:
Similar Structures:
Biological Unit for 2G9N: monomeric; determined by author
Molecular Components in 2G9N
Label Count Molecule
Protein (1 molecule)
Eukaryotic Initiation Factor 4a-i(Gene symbol: EIF4A1)
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB