2FGC: Crystal Structure Of Acetolactate Synthase- Small Subunit From Thermotoga Maritima

Citation:
Abstract
Crystal structures of two orthologs of the regulatory subunit of acetohydroxyacid synthase III (AHAS, EC 2.2.1.6) from Thermotoga maritima (TM0549) and Nitrosomonas europea (NE1324) were determined by single-wavelength anomalous diffraction methods with the use of selenomethionine derivatives at 2.3 A and 2.5 A, respectively. TM0549 and NE1324 share the same fold, and in both proteins the polypeptide chain contains two separate domains of a similar size. Each protein contains a C-terminal domain with ferredoxin-type fold and an N-terminal ACT domain, of which the latter is characteristic for several proteins involved in amino acid metabolism. The ferredoxin domain is stabilized by a calcium ion in the crystal structure of NE1324 and by a Mg(H2O)(6)2+ ion in TM0549. Both TM0549 and NE1324 form dimeric assemblies in the crystal lattice.
PDB ID: 2FGCDownload
MMDB ID: 37738
PDB Deposition Date: 2005/12/21
Updated in MMDB: 2006/12
Experimental Method:
x-ray diffraction
Resolution: 2.3  Å
Source Organism:
Similar Structures:
Biological Unit for 2FGC: dimeric; determined by author and by software (PISA,PQS)
Molecular Components in 2FGC
Label Count Molecule
Proteins (2 molecules)
2
Acetolactate Synthase, Small Subunit(Gene symbol: ilvH)
Molecule annotation
Chemicals (2 molecules)
1
2
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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