2F6M: Structure Of A Vps23-C:vps28-N Subcomplex

The endosomal sorting complex required for transport (ESCRT) complexes are central to receptor downregulation, lysosome biogenesis, and budding of HIV. The yeast ESCRT-I complex contains the Vps23, Vps28, and Vps37 proteins, and its assembly is directed by the C-terminal steadiness box of Vps23, the N-terminal half of Vps28, and the C-terminal half of Vps37. The crystal structures of a Vps23:Vps28 core subcomplex and the Vps23:Vps28:Vps37 core were solved at 2.1 and 2.8 A resolution. Each subunit contains a structurally similar pair of helices that form the core. The N-terminal domain of Vps28 has a hydrophobic binding site on its surface that is conformationally dynamic. The C-terminal domain of Vps28 binds the ESCRT-II complex. The structure shows how ESCRT-I is assembled by a compact core from which the Vps23 UEV domain, the Vps28 C domain, and other domains project to bind their partners.
PDB ID: 2F6MDownload
MMDB ID: 38716
PDB Deposition Date: 2005/11/29
Updated in MMDB: 2012/12
Experimental Method:
x-ray diffraction
Resolution: 2.1  Å
Source Organism:
Similar Structures:
Biological Unit for 2F6M: dimeric; determined by author and by software (PISA)
Molecular Components in 2F6M
Label Count Molecule
Proteins (2 molecules)
Suppressor Protein Stp22 of Temperature-sensitive Alpha- Factor Receptor and Arginine Permease(Gene symbol: STP22)
Molecule annotation
Vacuolar Protein Sorting-associated Protein Vps28(Gene symbol: VPS28)
Molecule annotation
Chemicals (5 molecules)
* Click molecule labels to explore molecular sequence information.

Citing MMDB