2F69: Ternary Complex Of Set79 BOUND TO ADOHCY AND A TAF10 Peptide

Human SET7/9 is a protein lysine methyltransferase (PKMT) that methylates histone H3, the tumor suppressor p53 and the TBP-associated factor TAF10. To elucidate the determinants of its substrate specificity, we have solved the enzyme's structure bound to a TAF10 peptide and examined its ability to methylate histone H3, TAF10 and p53 substrates bearing either mutations or covalent modifications within their respective methylation sites. Collectively, our data reveal that SET7/9 recognizes a conserved K/R-S/T/A motif preceding the lysine substrate and has a propensity to bind aspartates and asparagines on the C-terminal side of the lysine target. We then used a sequence-based approach with this motif to identify novel substrates for this PKMT. Among the putative targets is TAF7, which is methylated at Lys5 by the enzyme in vitro. These results demonstrate the predictive value of the consensus motif in identifying novel substrates for SET7/9.
PDB ID: 2F69Download
MMDB ID: 103666
PDB Deposition Date: 2005/11/28
Updated in MMDB: 2012/10
Experimental Method:
x-ray diffraction
Resolution: 1.3  Å
Source Organism:
synthetic construct
Similar Structures:
Biological Unit for 2F69: dimeric; determined by author and by software (PISA)
Molecular Components in 2F69
Label Count Molecule
Proteins (2 molecules)
Histone-lysine N-methyltransferase, H3 Lysine-4 Specific Set7(Gene symbol: SETD7)
Molecule annotation
Taf10 Peptide, Acetyl-ser-lys-ser-mlz-asp-arg- Lys-tyr-thr-leu
Molecule annotation
Chemical (1 molecule)
* Click molecule labels to explore molecular sequence information.

Citing MMDB