2EZE: SOLUTION STRUCTURE OF A COMPLEX OF THE SECOND DNA BINDING DOMAIN OF HUMAN HMG-I(Y) BOUND TO DNA DODECAMER CONTAINING THE PRDII SITE OF THE INTERFERON-BETA PROMOTER, NMR, 35 STRUCTURES

Citation:
Abstract
The solution structure of a complex between a truncated form of HMG-I(Y), consisting of the second and third DNA binding domains (residues 51-90), and a DNA dodecamer containing the PRDII site of the interferon-beta promoter has been solved by multidimensional nuclear magnetic resonance spectroscopy. The stoichiometry of the complex is one molecule of HMG-I(Y) to two molecules of DNA. The structure reveals a new architectural minor groove binding motif which stabilizes B-DNA, thereby facilitating the binding of other transcription factors in the opposing major groove. The interactions involve a central Arg-Gly-Arg motif together with two other modules that participate in extensive hydrophobic and polar contracts. The absence of one of these modules in the third DNA binding domain accounts for its-100 fold reduced affinity relative to the second one.
PDB ID: 2EZEDownload
MMDB ID: 53111
PDB Deposition Date: 1997/6/4
Updated in MMDB: 2012/11
Experimental Method:
solution nmr
Source Organism:
Homo sapiens
Similar Structures:
Molecular Components in 2EZE
Label Count Molecule
Protein (1 molecule)
1
High Mobility Group Protein Hmg-i/hmg-y(Gene symbol: HMGA1)
Molecule annotation
Nucleotides(2 molecules)
1
DNA (5'-d(*gp*gp*gp*ap*ap*ap*tp*tp*cp*cp*tp*c)-3')
Molecule annotation
1
DNA (5'-d(*gp*ap*gp*gp*ap*ap*tp*tp*tp*cp*cp*c)-3')
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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