2EYV: SH2 domain of CT10-Regulated Kinase

CRKI (SH2-SH3) and CRKII (SH2-SH3-SH3) are splicing isoforms of the oncoprotein CRK that regulate transcription and cytoskeletal reorganization for cell growth and motility by linking tyrosine kinases to small G proteins. CRKI shows substantial transforming activity, whereas the activity of CRKII is low, and phosphorylated CRKII has no biological activity whatsoever. The molecular mechanisms underlying the distinct biological activities of the CRK proteins remain elusive. We determined the solution structures of CRKI, CRKII and phosphorylated CRKII by NMR and identified the molecular mechanism that gives rise to their activities. Results from mutational analysis using rodent 3Y1 fibroblasts were consistent with those from the structural studies. Together, these data suggest that the linker region modulates the binding of CRKII to its targets, thus regulating cell growth and motility.
PDB ID: 2EYVDownload
MMDB ID: 46861
PDB Deposition Date: 2005/11/10
Updated in MMDB: 2007/11
Experimental Method:
solution nmr
Source Organism:
Similar Structures:
Molecular Components in 2EYV
Label Count Molecule
Protein (1 molecule)
V-crk Sarcoma Virus Ct10 Oncogene Homolog Isoform a(Gene symbol: CRK)
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB