2EQQ: Solution structure of growth-blocking peptide of the armyworm, Pseudaletia separata

Growth-blocking peptide (GBP) is a hormone-like peptide that suppresses the growth of the host armyworm. Although the 23-amino acid GBP (1-23 GBP) is expressed in nonparasitized armyworm plasma, the parasitization by wasp produces the 28-amino acid GBP (1-28 GBP) through an elongation of the C-terminal amino acid sequence. In this study, we characterized the GBP variants, which consist of various lengths of the C-terminal region, by comparing their biological activities and three-dimensional structures. The results of an injection study indicate that 1-28 GBP most strongly suppresses larval growth. NMR analysis shows that these peptides have basically the same tertiary structures and that the extension of the C-terminal region is disordered. However, the C-terminal region of 1-28 GBP undergoes a conformational transition from a random coiled state to an alpha-helical state in the presence of dodecylphosphocholine micelles. This suggests that binding of the C-terminal region would affect larval growth activity.
PDB ID: 2EQQDownload
MMDB ID: 63389
PDB Deposition Date: 2007/3/30
Updated in MMDB: 2009/07
Experimental Method:
solution nmr
Source Organism:
Similar Structures:
Biological Unit for 2EQQ: monomeric; determined by author
Molecular Components in 2EQQ
Label Count Molecule
Protein (1 molecule)
Growth-blocking Peptide, Long Form
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB