2EHO: Crystal Structure Of Human Gins Complex

The GINS complex mediates the assembly of the MCM2-7 (minichromosome maintenance) complex with proteins in a replisome progression complex. The eukaryotic GINS complex is composed of Sld5, Psf1, Psf2, and Psf3, which must be assembled for cell proliferation. We determined the crystal structure of the human GINS complex: GINS forms an elliptical shape with a small central channel. The structures of Sld5 and Psf2 resemble those of Psf1 and Psf3, respectively. In addition, the N-terminal and C-terminal domains of Sld5/Psf1 are permuted in Psf2/Psf3, which suggests that the four proteins have evolved from a common ancestor. Using a structure-based mutational analysis, we identified the functionally critical surface regions of the GINS complex.
PDB ID: 2EHODownload
MMDB ID: 46893
PDB Deposition Date: 2007/3/7
Updated in MMDB: 2012/11
Experimental Method:
x-ray diffraction
Resolution: 3  Å
Source Organism:
Similar Structures:
Biological Unit for 2EHO: tetrameric; determined by author and by software (PISA)
Molecular Components in 2EHO
Label Count Molecule
Proteins (4 molecules)
Gins Complex Subunit 4(Gene symbol: GINS4)
Molecule annotation
DNA Replication Complex Gins Protein Psf1(Gene symbol: GINS1)
Molecule annotation
DNA Replication Complex Gins Protein Psf2(Gene symbol: GINS2)
Molecule annotation
Gins Complex Subunit 3(Gene symbol: GINS3)
Molecule annotation
Chemicals (3 molecules)
* Click molecule labels to explore molecular sequence information.

Citing MMDB