2EGH: Crystal Structure Of 1-Deoxy-D-Xylulose 5-Phosphate Reductoisomerase Complexed With A Magnesium Ion, Nadph And Fosmidomycin

Citation:
Abstract
The crystal structure of 1-deoxy-D-xylulose 5-phosphate reductoisomerase (DXR) from Escherichia coli complexed with Mg(2+), NADPH and fosmidomycin was solved at 2.2 A resolution. DXR is the key enzyme in the 2-C-methyl-D-erythritol 4-phosphate pathway and is an effective target of antimalarial drugs such as fosmidomycin. In the crystal structure, electron density for the flexible loop covering the active site was clearly observed, indicating the well ordered conformation of DXR upon substrate binding. On the other hand, no electron density was observed for the nicotinamide-ribose portion of NADPH and the position of Asp149 anchoring Mg(2+) was shifted by NADPH in the active site.
PDB ID: 2EGHDownload
MMDB ID: 46892
PDB Deposition Date: 2007/3/1
Updated in MMDB: 2007/11
Experimental Method:
x-ray diffraction
Resolution: 2.2  Å
Source Organism:
Similar Structures:
Biological Unit for 2EGH: dimeric; determined by author and by software (PISA)
Molecular Components in 2EGH
Label Count Molecule
Proteins (2 molecules)
2
1-deoxy-d-xylulose 5-phosphate Reductoisomerase(Gene symbol: dxr)
Molecule annotation
Chemicals (6 molecules)
1
2
2
2
3
2
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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