2E2S: Solution structure of agelenin, an insecticidal peptide from the venom of Agelena opulenta

Agelenin, isolated from the Agelenidae spider Agelena opulenta, is a peptide composed of 35 amino acids. We determined the three-dimensional structure of agelenin using two-dimensional NMR spectroscopy. The structure is composed of a short antiparallel beta-sheet and four beta-turns, which are stabilized by three disulfide bonds. Agelenin has characteristic residues, Phe9, Ser28 and Arg33, which are arranged similarly to the pharmacophore of the insect channel inhibitor, omega-atracotoxin-Hv1a. These observations suggest that agelenin and omega-atracotoxin-Hv1a bind to insect calcium channels in a similar manner. We also suggest that another mode of action may operate in the channel inhibition by omega-agatoxin-IVA and omega-atracotoxin-Hv2a.
PDB ID: 2E2SDownload
MMDB ID: 74597
PDB Deposition Date: 2006/11/17
Updated in MMDB: 2009/07
Experimental Method:
solution nmr
Source Organism:
Similar Structures:
Molecular Components in 2E2S
Label Count Molecule
Protein (1 molecule)
Molecule annotation
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