2E2I: Rna Polymerase Ii Elongation Complex In 5 Mm Mg+2 With 2'- Dgtp

Citation:
Abstract
New structures of RNA polymerase II (pol II) transcribing complexes reveal a likely key to transcription. The trigger loop swings beneath a correct nucleoside triphosphate (NTP) in the nucleotide addition site, closing off the active center and forming an extensive network of interactions with the NTP base, sugar, phosphates, and additional pol II residues. A histidine side chain in the trigger loop, precisely positioned by these interactions, may literally "trigger" phosphodiester bond formation. Recognition and catalysis are thus coupled, ensuring the fidelity of transcription.
PDB ID: 2E2IDownload
MMDB ID: 107389
PDB Deposition Date: 2006/11/14
Updated in MMDB: 2013/02
Experimental Method:
x-ray diffraction
Resolution: 3.41  Å
Source Organism:
synthetic construct
Similar Structures:
Biological Unit for 2E2I: tridecameric; determined by author
Molecular Components in 2E2I
Label Count Molecule
Proteins (10 molecules)
1
DNA-directed RNA Polymerase II Largest Subunit(Gene symbol: RPO21)
Molecule annotation
1
DNA-directed RNA Polymerase II 140 KDA Polypeptide(Gene symbol: RPB2)
Molecule annotation
1
DNA-directed RNA Polymerase II 45 KDA Polypeptide(Gene symbol: RPB3)
Molecule annotation
1
DNA-directed RNA Polymerases I, II, and III 27 KDA Polypeptide(Gene symbol: RPB5)
Molecule annotation
1
DNA-directed RNA Polymerases I, II, and III 23 KDA Polypeptide(Gene symbol: RPO26)
Molecule annotation
1
DNA-directed RNA Polymerases I, II, and III 14.5 KDA Polypeptide(Gene symbol: RPB8)
Molecule annotation
1
DNA-directed RNA Polymerase II Subunit 9(Gene symbol: RPB9)
Molecule annotation
1
DNA-directed RNA Polymerases I/ii/iii Subunit 10(Gene symbol: RPB10)
Molecule annotation
1
DNA-directed RNA Polymerase II 13.6 KDA Polypeptide(Gene symbol: RPB11)
Molecule annotation
1
DNA-directed RNA Polymerases I, II, and III 7.7 KDA Polypeptide(Gene symbol: RPC10)
Molecule annotation
Nucleotides(3 molecules)
1
5'-r(*ap*up*cp*gp*ap*gp*ap*gp*gp*a)-3'
Molecule annotation
1
28-mer DNA Template Strand
Molecule annotation
1
5'-d(*cp*tp*gp*cp*tp*tp*ap*tp*cp*gp*gp*tp*ap*g)- 3'
Molecule annotation
Chemicals (10 molecules)
1
8
2
1
3
1
* Click molecule labels to explore molecular sequence information.

Citing MMDB
.