2DXC: Recombinant Thiocyanate Hydrolase, Fully-Matured Form

Citation:
Abstract
Thiocyanate hydrolase (SCNase) is a member of a family of nitrile hydratase proteins, each of which contains a unique noncorrin cobalt center with two post-translationally modified cysteine ligands, cysteine-sulfenic acid or -sulfenate (Cys-SO(H)), and cysteine-sulfininate (Cys-SO(2)(-)), respectively. We have found that a partially matured recombinant SCNase was activated during storage. The crystal structures of SCNase before and after storage demonstrated that Cys-SO(2)(-) modification of gammaCys131 proceeded to completion prior to storage, while Cys-SO(H) modification of gammaCys133 occurred during storage. SCNase activity was suppressed when gammaCys133 was further oxidized to Cys-SO(2)(-). The correlation between the catalytic activity and the extent of the gammaCys133 modification indicates that the cysteine sulfenic acid modification of gammaCys133 is of primary importance in determining the activity of SCNase.
PDB ID: 2DXCDownload
MMDB ID: 58650
PDB Deposition Date: 2006/8/25
Updated in MMDB: 2012/11
Experimental Method:
x-ray diffraction
Resolution: 1.9  Å
Source Organism:
Similar Structures:
Biological Unit for 2DXC: dodecameric; determined by author and by software (PISA,PQS)
Molecular Components in 2DXC
Label Count Molecule
Proteins (12 molecules)
4
Thiocyanate Hydrolase Subunit Alpha
Molecule annotation
4
Thiocyanate Hydrolase Subunit Beta
Molecule annotation
4
Thiocyanate Hydrolase Subunit Gamma
Molecule annotation
Chemicals (8 molecules)
1
4
2
4
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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