2D6Y: Crystal Structure Of Transcriptional Factor Sco4008 From Streptomyces Coelicolor A3(2)

Citation:
Abstract
SCO4008 from Streptomyces coelicolor A3(2) is a member of the TetR family. However, its precise function is not yet clear. In this study, the crystal structure of SCO4008 was determined at a resolution of 2.3A, and its DNA-binding properties were analyzed. Crystal structure analysis showed that SCO4008 forms an Omega-shaped homodimer in which the monomer is composed of an N-terminal DNA-binding domain containing a helix-turn-helix and a C-terminal dimerization and regulatory domain possessing a ligand-binding cavity. The genomic systematic evolution of ligands by exponential enrichment and electrophoretic mobility shift assay revealed that four SCO4008 dimers bind to the two operator regions located between sco4008 and sco4007, a secondary transporter belonging to the major facilitator superfamily. Ligand screening analysis showed that SCO4008 recognizes a wide range of structurally dissimilar cationic and hydrophobic compounds. These results suggested that SCO4008 is a transcriptional repressor of sco4007 responsible for the multidrug resistance system in S. coelicolor A3(2).
PDB ID: 2D6YDownload
MMDB ID: 41985
PDB Deposition Date: 2005/11/15
Updated in MMDB: 2012/11
Experimental Method:
x-ray diffraction
Resolution: 2.3  Å
Source Organism:
Similar Structures:
Biological Unit for 2D6Y: dimeric; determined by author and by software (PISA)
Molecular Components in 2D6Y
Label Count Molecule
Proteins (2 molecules)
2
Putative Tetr Family Regulatory Protein(Gene symbol: SCO4008)
Molecule annotation
Chemicals (2 molecules)
1
2
* Click molecule labels to explore molecular sequence information.

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