2CSB: Crystal Structure Of Topoisomerase V From Methanopyrus Kandleri (61 Kda Fragment)

Topoisomerases are involved in controlling and maintaining the topology of DNA and are present in all kingdoms of life. Unlike all other types of topoisomerases, similar type IB enzymes have only been identified in bacteria and eukarya. The only putative type IB topoisomerase in archaea is represented by Methanopyrus kandleri topoisomerase V. Despite several common functional characteristics, topoisomerase V shows no sequence similarity to other members of the same type. The structure of the 61 kDa N-terminal fragment of topoisomerase V reveals no structural similarity to other topoisomerases. Furthermore, the structure of the active site region is different, suggesting no conservation in the cleavage and religation mechanism. Additionally, the active site is buried, indicating the need of a conformational change for activity. The presence of a topoisomerase in archaea with a unique structure suggests the evolution of a separate mechanism to alter DNA.
PDB ID: 2CSBDownload
MMDB ID: 37236
PDB Deposition Date: 2005/5/21
Updated in MMDB: 2017/11
Experimental Method:
x-ray diffraction
Resolution: 2.3  Å
Source Organism:
Similar Structures:
Biological Unit for 2CSB: monomeric; determined by author
Molecular Components in 2CSB
Label Count Molecule
Protein (1 molecule)
Topoisomerase V
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB