2BLD: The Quasi-atomic Model Of Human Adenovirus Type 5 Capsid (part 1)

Citation:
Abstract
Adenoviruses infect a wide range of vertebrates including humans. Their icosahedral capsids are composed of three major proteins: the trimeric hexon forms the facets and the penton, a noncovalent complex of the pentameric penton base and trimeric fibre proteins, is located at the 12 capsid vertices. Several proteins (IIIa, VI, VIII and IX) stabilise the capsid. We have obtained a 10 A resolution map of the human adenovirus 5 by image analysis from cryo-electron micrographs (cryoEMs). This map, in combination with the X-ray structures of the penton base and hexon, was used to build a quasi-atomic model of the arrangement of the two major capsid components and to analyse the hexon-hexon and hexon-penton interactions. The secondary proteins, notably VIII, were located by comparing cryoEM maps of native and pIX deletion mutant virions. Minor proteins IX and IIIa are located on the outside of the capsid, whereas protein VIII is organised with a T=2 lattice on the inner face of the capsid. The capsid organisation is compared with the known X-ray structure of bacteriophage PRD1.
MMDB ID: 99466
PDB Deposition Date: 2005/3/3
Updated in MMDB: 2012/08
Experimental Method:
electron microscopy
Resolution: 10  Å
Source Organism:
Human adenovirus 5
Similar Structures:
Merged PDB IDs:2BLD 2BVI
Biological Unit for 2BLD: 22-meric
Molecular Components in 2BLD
Label Count Molecule
Proteins (22 molecules)
5
Penton Protein
Molecule annotation
5
N-terminal Peptide of Fiber Protein
Molecule annotation
12
Hexon Protein
Molecule annotation
* Click molecule labels to explore molecular sequence information.

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