2AZ1: Structure Of A Halophilic Nucleoside Diphosphate Kinase From Halobacterium Salinarum

Nucleoside diphosphate kinase from the halophilic archaeon Halobacterium salinarum was crystallized in a free state and a substrate-bound form with CDP. The structures were solved to a resolution of 2.35 and 2.2A, respectively. Crystals with the apo-form were obtained with His6-tagged enzyme, whereas the untagged form was used for co-crystallization with the nucleotide. Crosslinking under different salt and pH conditions revealed a stronger oligomerization tendency for the tagged protein at low and high salt concentrations. The influence of the His6-tag on the halophilic nature of the enzyme is discussed on the basis of the observed structural properties.
PDB ID: 2AZ1Download
MMDB ID: 36696
PDB Deposition Date: 2005/9/9
Updated in MMDB: 2017/11
Experimental Method:
x-ray diffraction
Resolution: 2.35  Å
Source Organism:
Similar Structures:
Biological Unit for 2AZ1: hexameric; determined by author and by software (PISA)
Molecular Components in 2AZ1
Label Count Molecule
Proteins (6 molecules)
Nucleoside Diphosphate Kinase(Gene symbol: VNG_RS04530)
Molecule annotation
Chemicals (5 molecules)
* Click molecule labels to explore molecular sequence information.

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