2AQF: Structural and functional analysis of ADA2 alpha swirm domain

The evolutionarily conserved Swi3p, Rsc8p and Moira (SWIRM) domain is found in many chromosomal proteins involved in chromatin modifications or remodeling. Here we report the three-dimensional solution structure of the SWIRM domain from the human transcriptional adaptor ADA2alpha. The structure reveals a five-helix bundle consisting of two helix-turn-helix motifs connected by a central long helix, reminiscent of the histone fold. Using structural and biochemical analyses, we showed that the SWIRM domains of human ADA2alpha and SMARC2 bind to double-stranded and nucleosomal DNA, and we identified amino acid residues required for this function. We demonstrated that the ADA2alpha SWIRM domain is colocalized with lysine-acetylated histone H3 in the cell nucleus and that it potentiates the ACF remodeling activity by enhancing accessibility of nucleosomal linker DNA bound to histone H1. These data suggest a functional role of the SWIRM domain in chromatin remodeling.
PDB ID: 2AQFDownload
MMDB ID: 37080
PDB Deposition Date: 2005/8/17
Updated in MMDB: 2006/12
Experimental Method:
solution nmr
Source Organism:
Similar Structures:
Molecular Components in 2AQF
Label Count Molecule
Protein (1 molecule)
Transcriptional Adaptor 2, Ada2 Alpha(Gene symbol: Tada2a)
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB