2APC: Crystal Structure Of N-acetylglucosaminyltransferase I In Complex With Udp-glcnac Phosphonate

Citation:
Abstract
The Golgi-resident glycosyltransferase, UDP-N-acetyl-d-glucosamine:alpha-3-d-mannoside beta-1,2-N-acetylglucosaminyltransferase I (GnT I), initiates the conversion of high-mannose oligosaccharides to complex and hybrid structures in the biosynthesis of N-linked glycans. Reported here are the X-ray crystal structures of GnT I in complex with UDP-CH2-GlcNAc (a non-hydrolyzable C-glycosidic phosphonate), UDP-2-deoxy-2-fluoro-glucose, UDP-glucose and UDP. Collectively, these structures provide evidence for the importance of the GlcNAc moiety and its N-acetyl group in donor substrate binding, as well as insight into the role played by the flexible 318-330 loop in substrate binding and product release. In addition, the UDP-CH2-GlcNAc complex reveals a well-defined glycerol molecule poised for nucleophilic attack on the C1 atom of the donor substrate analogue. The position and orientation of this glycerol molecule have allowed us to model the binding of the Manalpha1,3Manbeta1 moiety of the acceptor substrate and, based on the model, to suggest a rationalization for the main determinants of GnT I acceptor specificity.
PDB ID: 2APCDownload
MMDB ID: 39574
PDB Deposition Date: 2005/8/16
Updated in MMDB: 2007/10
Experimental Method:
x-ray diffraction
Resolution: 1.5  Å
Source Organism:
Similar Structures:
Biological Unit for 2APC: monomeric; determined by author
Molecular Components in 2APC
Label Count Molecule
Protein (1 molecule)
1
Alpha-1,3-mannosyl-glycoprotein 2-beta-n- Acetylglucosaminyltransferase(Gene symbol: MGAT1)
Molecule annotation
Chemicals (8 molecules)
1
1
2
1
3
6
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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