2ADC: Solution structure of Polypyrimidine Tract Binding protein RBD34 complexed with CUCUCU RNA

Citation:
Abstract
The polypyrimidine tract binding protein (PTB) is a 58-kilodalton RNA binding protein involved in multiple aspects of messenger RNA metabolism, including the repression of alternative exons. We have determined the solution structures of the four RNA binding domains (RBDs) of PTB, each bound to a CUCUCU oligonucleotide. Each RBD binds RNA with a different binding specificity. RBD3 and RBD4 interact, resulting in an antiparallel orientation of their bound RNAs. Thus, PTB will induce RNA looping when bound to two separated pyrimidine tracts within the same RNA. This leads to structural models for how PTB functions as an alternative-splicing repressor.
PDB ID: 2ADCDownload
MMDB ID: 35190
PDB Deposition Date: 2005/7/20
Updated in MMDB: 2007/10
Experimental Method:
solution nmr
Source Organism:
Homo sapiens
Similar Structures:
Molecular Components in 2ADC
Label Count Molecule
Protein (1 molecule)
1
Polypyrimidine Tract-binding Protein 1(Gene symbol: PTBP1)
Molecule annotation
Nucleotide(1 molecule)
2
5'-r(*cp*up*cp*up*cp*u)-3'
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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