1Z0T: Crystal Structure Of A. Fulgidus Lon Proteolytic Domain

Although macromolecular crystallography is rapidly becoming largely routine owing to advances in methods of data collection, structure solution and refinement, difficult cases are still common. To remind structural biologists about the kinds of crystallographic difficulties that might be encountered, case studies of several successfully completed structure determinations that utilized less than perfect crystals are discussed here. The structure of the proteolytic domain of Archaeoglobus fulgidus Lon was solved with crystals that contained superimposed orthorhombic and monoclinic lattices, a case not previously described for proteins. Another hexagonal crystal form of this protein exhibited an unusually high degree of non-isomorphism. Crystals of A. fulgidus Rio1 kinase exhibited both pseudosymmetry and twinning. Ways of identifying the observed phenomena and approaches to solving and refining macromolecular structures when only less than perfect crystals are available are discussed here.
PDB ID: 1Z0TDownload
MMDB ID: 34271
PDB Deposition Date: 2005/3/2
Updated in MMDB: 2017/11
Experimental Method:
x-ray diffraction
Resolution: 3  Å
Source Organism:
Similar Structures:
Biological Unit for 1Z0T: hexameric; determined by author
Molecular Components in 1Z0T
Label Count Molecule
Proteins (6 molecules)
Putative Protease LA Homolog Type(Gene symbol: AF_RS01865)
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB