1YYO: Crystal Structure Of Rnase Iii Mutant E110k From Aquifex Aeolicus Complexed With Double-stranded Rna At 2.9- Angstrom Resolution

Bacterial ribonuclease III (RNase III) can affect RNA structure and gene expression in either of two ways: as a processing enzyme that cleaves double-stranded (ds) RNA, or as a binding protein that binds but does not cleave dsRNA. We previously proposed a model of the catalytic complex of RNase III with dsRNA based on three crystal structures, including the endonuclease domain of RNase III with and without bound metal ions and a dsRNA binding protein complexed with dsRNA. We also reported a noncatalytic assembly observed in the crystal structure of an RNase III mutant, which binds but does not cleave dsRNA, complexed with dsRNA. We hypothesize that the RNase III*dsRNA complex can exist in two functional forms, a catalytic complex and a noncatalytic assembly, and that in between the two forms there may be intermediate states. Here, we present four crystal structures of RNase III complexed with dsRNA, representing possible intermediates.
PDB ID: 1YYODownload
MMDB ID: 107017
PDB Deposition Date: 2005/2/25
Updated in MMDB: 2013/02
Experimental Method:
x-ray diffraction
Resolution: 2.9  Å
Source Organism:
Aquifex aeolicus
Similar Structures:
Biological Unit for 1YYO: hexameric; determined by author
Molecular Components in 1YYO
Label Count Molecule
Proteins (2 molecules)
Ribonuclease III(Gene symbol: rnc)
Molecule annotation
Nucleotide(1 molecule)
Molecule annotation
Chemical (1 molecule)
* Click molecule labels to explore molecular sequence information.

Citing MMDB