1YP1: Crystal Structure Of A Non-hemorrhagic Fibrin(ogen)olytic Metalloproteinase From Venom Of Agkistrodon Acutus

Citation:
Abstract
Thrombotic occlusive diseases pose a great threat to human health. Thrombolytic agents are in widespread use for the dissolution of arterial and venous pathologic thrombi in these kinds of diseases. Snake venom metalloproteinases (SVMPs) can act directly on fibrin/fibrinogen and are therefore potential candidates for therapeutic use against thrombotic occlusive diseases. In this study, we have determined the crystal structure of FII, a novel non-hemorrhagic SVMP isolated from Anhui Agkistrodon acutus snake venom by molecular replacement. The structure reveals that FII is a member of the P-I class SVMPs. The Zn2+ ion essential for hydrolytic activity is found in the active site and is tetrahedrally co-ordinated by three histidine residues and water molecule. Unambiguous electron density for a tri-peptide with sequence KNL is also found located near the active site. Biochemical evidences show that the tri-peptide KNL can inhibit the enzymatic activity of FII.
PDB ID: 1YP1Download
MMDB ID: 36975
PDB Deposition Date: 2005/1/28
Updated in MMDB: 2007/10
Experimental Method:
x-ray diffraction
Resolution: 1.9  Å
Source Organism:
Deinagkistrodon acutus
Similar Structures:
Biological Unit for 1YP1: dimeric; determined by author and by software (PISA)
Molecular Components in 1YP1
Label Count Molecule
Proteins (2 molecules)
1
FII
Molecule annotation
1
KNL
Molecule annotation
Chemical (1 molecule)
1
1
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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