1Y4Z: The Crystal Structure Of Nitrate Reductase A, Narghi, In Complex With The Q-Site Inhibitor Pentachlorophenol

Citation:
Abstract
The crystal structure of Escherichia coli nitrate reductase A (NarGHI) in complex with pentachlorophenol has been determined to 2.0 A of resolution. We have shown that pentachlorophenol is a potent inhibitor of quinol:nitrate oxidoreductase activity and that it also perturbs the EPR spectrum of one of the hemes located in the membrane anchoring subunit (NarI). This new structural information together with site-directed mutagenesis data, biochemical analyses, and molecular modeling provide the first molecular characterization of a quinol binding and oxidation site (Q-site) in NarGHI. A possible proton conduction pathway linked to electron transfer reactions has also been defined, providing fundamental atomic details of ubiquinol oxidation by NarGHI at the bacterial membrane.
PDB ID: 1Y4ZDownload
MMDB ID: 32483
PDB Deposition Date: 2004/12/1
Updated in MMDB: 2012/10
Experimental Method:
x-ray diffraction
Resolution: 2  Å
Source Organism:
Similar Structures:
Biological Unit for 1Y4Z: trimeric; determined by author and by software (PISA)
Molecular Components in 1Y4Z
Label Count Molecule
Proteins (3 molecules)
1
Respiratory Nitrate Reductase 1 Alpha Chain(Gene symbol: narG)
Molecule annotation
1
Respiratory Nitrate Reductase 1 Beta Chain(Gene symbol: narH)
Molecule annotation
1
Respiratory Nitrate Reductase 1 Gamma Chain(Gene symbol: narI)
Molecule annotation
Chemicals (12 molecules)
1
2
2
1
3
3
4
1
5
2
6
2
7
1
* Click molecule labels to explore molecular sequence information.

Citing MMDB
.