1Y4J: Crystal Structure Of The Paralogue Of The Human Formylglycine Generating Enzyme

Citation:
Abstract
In eukaryotes, sulfate esters are degraded by sulfatases, which possess a unique Calpha-formylglycine residue in their active site. The defect in post-translational formation of the Calpha-formylglycine residue causes a severe lysosomal storage disorder in humans. Recently, FGE (formylglycine-generating enzyme) has been identified as the protein required for this specific modification. Using sequence comparisons, a protein homologous to FGE was found and denoted pFGE (paralog of FGE). pFGE binds a sulfatase-derived peptide bearing the FGE recognition motif, but it lacks formylglycine-generating activity. Both proteins belong to a large family of pro- and eukaryotic proteins containing the DUF323 domain, a formylglycine-generating enzyme domain of unknown three-dimensional structure. We have crystallized the glycosylated human pFGE and determined its crystal structure at a resolution of 1.86 A. The structure reveals a novel fold, which we denote the FGE fold and which therefore serves as a paradigm for the DUF323 domain. It is characterized by an asymmetric partitioning of secondary structure elements and is stabilized by two calcium cations. A deep cleft on the surface of pFGE most likely represents the sulfatase polypeptide binding site. The asymmetric unit of the pFGE crystal contains a homodimer. The putative peptide binding site is buried between the monomers, indicating a biological significance of the dimer. The structure suggests the capability of pFGE to form a heterodimer with FGE.
PDB ID: 1Y4JDownload
MMDB ID: 32027
PDB Deposition Date: 2004/12/1
Updated in MMDB: 2014/11
Experimental Method:
x-ray diffraction
Resolution: 1.86  Å
Source Organism:
Similar Structures:
Biological Unit for 1Y4J: dimeric; determined by author and by software (PISA)
Molecular Components in 1Y4J
Label Count Molecule
Proteins (2 molecules)
2
Sulfatase Modifying Factor 2(Gene symbol: SUMF2)
Molecule annotation
Chemicals (10 molecules)
1
4
2
1
3
4
4
1
* Click molecule labels to explore molecular sequence information.

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