1XT6: S35c Flavodoxin Mutant In The Semiquinone State

Citation:
Abstract
The crystallographic structure of an engineered flavodoxin mutant from Desulfovibrio vulgaris has been analysed. Site-directed mutagenesis was used to substitute serine 35 with a cysteine to provide a possible covalent linkage. The crystal structure of the semiquinone form of this mutant is similar to the corresponding oxidation state of the wild-type flavodoxin. Analysis of the structural changes reveals the interaction between N(5)H of the flavin and the carbonyl O atom of Gly61 to be critical for modulation of the electrochemical properties of the protein.
PDB ID: 1XT6Download
MMDB ID: 31660
PDB Deposition Date: 2004/10/21
Updated in MMDB: 2007/11
Experimental Method:
x-ray diffraction
Resolution: 1.8  Å
Source Organism:
Similar Structures:
Biological Unit for 1XT6: monomeric; determined by author
Molecular Components in 1XT6
Label Count Molecule
Protein (1 molecule)
1
Flavodoxin(Gene symbol: DVU2680)
Molecule annotation
Chemical (1 molecule)
1
1
* Click molecule labels to explore molecular sequence information.

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