1XSF: Solution structure of a resuscitation promoting factor domain from Mycobacterium tuberculosis

Resuscitation-promoting factor (RPF) proteins reactivate stationary-phase cultures of (G+C)-rich Gram-positive bacteria including the causative agent of tuberculosis, Mycobacterium tuberculosis. We report the solution structure of the RPF domain from M. tuberculosis Rv1009 (RpfB) solved by heteronuclear multidimensional NMR. Structural homology with various glycoside hydrolases suggested that RpfB cleaved oligosaccharides. Biochemical studies indicate that a conserved active site glutamate is important for resuscitation activity. These data, as well as the presence of a clear binding pocket for a large molecule, indicate that oligosaccharide cleavage is probably the signal for revival from dormancy.
PDB ID: 1XSFDownload
MMDB ID: 31988
PDB Deposition Date: 2004/10/19
Updated in MMDB: 2007/11
Experimental Method:
solution nmr
Source Organism:
Similar Structures:
Molecular Components in 1XSF
Label Count Molecule
Protein (1 molecule)
Probable Resuscitation-promoting Factor Rpfb
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB