1WO6: Solution structure of Designed Functional Finger 5 (DFF5): Designed mutant based on non-native CHANCE domain

Zinc binding motifs have received much attention in the area of protein design. Here, we have tested the suitability of a recently discovered nonnative zinc binding structure as a protein design scaffold. A series of multiple alanine mutants was created to investigate the minimal requirements for folding, and solution structures of these mutants showed that the original fold was maintained, despite changes in approximately 50% of the sequence. We next attempted to transplant binding faces from chosen bimolecular interactions onto one of these mutants, and many of the resulting "chimeras" were shown to adopt a native-like fold. These results both highlight the robust nature of small zinc binding domains and underscore the complexity of designing functional proteins, even using such small, highly ordered scaffolds as templates.
PDB ID: 1WO6Download
MMDB ID: 32355
PDB Deposition Date: 2004/8/12
Updated in MMDB: 2007/10
Experimental Method:
solution nmr
Similar Structures:
Molecular Components in 1WO6
Label Count Molecule
Protein (1 molecule)
Creb Binding Protein(Gene symbol: CREBBP)
Molecule annotation
Chemical (1 molecule)
* Click molecule labels to explore molecular sequence information.

Citing MMDB