1VY8: Crystal Structure Of The E. Coli Crispr Rna-guided Surveillance Complex, Cascade

Citation:
Abstract
Clustered regularly interspaced short palindromic repeats (CRISPRs) are essential components of RNA-guided adaptive immune systems that protect bacteria and archaea from viruses and plasmids. In Escherichia coli, short CRISPR-derived RNAs (crRNAs) assemble into a 405 kDa multi-subunit surveillance complex called Cascade (CRISPR-associated complex for antiviral defense). Here, we present the 3.24 A resolution x-ray crystal structure of Cascade. Eleven proteins and a 61-nucleotide crRNA assemble into a sea-horse-shaped architecture that binds double-stranded DNA targets complementary to the crRNA-guide sequence. Conserved sequences on the 3'- and 5'-ends of the crRNA are anchored by proteins at opposite ends of the complex, while the guide sequence is displayed along a helical assembly of six interwoven subunits that present 5-nucleotide segments of the crRNA in pseudo A-form configuration. The structure of Cascade suggests a mechanism for assembly and provides insights into the mechanisms of target recognition.
PDB ID: 1VY8Download
MMDB ID: 122470
PDB Deposition Date: 2014/6/28
Updated in MMDB: 2014/08
Experimental Method:
x-ray diffraction
Resolution: 3.24  Å
Source Organism:
Escherichia coli K-12
Similar Structures:
Biological Unit for 1VY8: dodecameric; determined by author
Molecular Components in 1VY8
Label Count Molecule
Proteins (11 molecules)
2
Crispr System Cascade Subunit Casb
Molecule annotation
6
Crispr System Cascade Subunit Casc
Molecule annotation
1
Crispr System Cascade Subunit Casd
Molecule annotation
1
Crispr System Cascade Subunit Case
Molecule annotation
1
Crispr System Cascade Subunit Casa
Molecule annotation
Nucleotide(1 molecule)
1
Escherichia Coli Strain Ecor44 Cluster 1 Crispr Region
Molecule annotation
Chemical (1 molecule)
1
1
* Click molecule labels to explore molecular sequence information.

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