1VXX: Structures Of Yeast 80s Ribosome-trna Complexes In The Rotated And Non-rotated Conformations (class I - Non-rotated Ribosome With 2 Trnas, This Entry Contains The Large Ribosomal Subunit Rna)

Citation:
Abstract
The structural understanding of eukaryotic translation lags behind that of translation on bacterial ribosomes. Here, we present two subnanometer resolution structures of S. cerevisiae 80S ribosome complexes formed with either one or two tRNAs and bound in response to an mRNA fragment containing the Kozak consensus sequence. The ribosomes adopt two globally different conformations that are related to each other by the rotation of the small subunit. Comparison with bacterial ribosome complexes reveals that the global structures and modes of intersubunit rotation of the yeast ribosome differ significantly from those in the bacterial counterpart, most notably in the regions involving the tRNA, small ribosomal subunit, and conserved helix 69 of the large ribosomal subunit. The structures provide insight into ribosome dynamics implicated in tRNA translocation and help elucidate the role of the Kozak fragment in positioning an open reading frame during translation initiation in eukaryotes.
MMDB ID: 122451
PDB Deposition Date: 2014/5/29
Updated in MMDB: 2014/09
Experimental Method:
electron microscopy
Resolution: 6.3  Å
Source Organism:
Saccharomyces cerevisiae
Similar Structures:
Merged PDB IDs:1VXX 1VXY 1VXZ
Biological Unit for 1VXX: 84-meric
Molecular Components in 1VXX
Label Count Molecule
Proteins (77 molecules)
1
60S Ribosomal Protein L1
Molecule annotation
1
60S Ribosomal Protein L2
Molecule annotation
1
60S Ribosomal Protein L3
Molecule annotation
1
60S Ribosomal Protein L4
Molecule annotation
1
60S Ribosomal Protein L5
Molecule annotation
1
60S Ribosomal Protein L6
Molecule annotation
1
60S Ribosomal Protein L7
Molecule annotation
1
60S Ribosomal Protein L8
Molecule annotation
1
60S Ribosomal Protein L9
Molecule annotation
1
60S Ribosomal Protein L10
Molecule annotation
1
60S Ribosomal Protein L11
Molecule annotation
1
60S Ribosomal Protein L12
Molecule annotation
1
60S Ribosomal Protein L13
Molecule annotation
1
60S Ribosomal Protein L14
Molecule annotation
1
60S Ribosomal Protein L15
Molecule annotation
1
60S Ribosomal Protein L16
Molecule annotation
1
60S Ribosomal Protein L17
Molecule annotation
1
60S Ribosomal Protein L18
Molecule annotation
1
60S Ribosomal Protein L19
Molecule annotation
1
60S Ribosomal Protein L20
Molecule annotation
1
60S Ribosomal Protein L21
Molecule annotation
1
60S Ribosomal Protein L22
Molecule annotation
1
60S Ribosomal Protein L23
Molecule annotation
1
60S Ribosomal Protein L24
Molecule annotation
1
60S Ribosomal Protein L25
Molecule annotation
1
60S Ribosomal Protein L26
Molecule annotation
1
60S Ribosomal Protein L27
Molecule annotation
1
60S Ribosomal Protein L28
Molecule annotation
1
60S Ribosomal Protein L29
Molecule annotation
1
60S Ribosomal Protein L30
Molecule annotation
1
60S Ribosomal Protein L31
Molecule annotation
1
60S Ribosomal Protein L32
Molecule annotation
1
60S Ribosomal Protein L33
Molecule annotation
1
60S Ribosomal Protein L34
Molecule annotation
1
60S Ribosomal Protein L35
Molecule annotation
1
60S Ribosomal Protein L36
Molecule annotation
1
60S Ribosomal Protein L37
Molecule annotation
1
60S Ribosomal Protein L38
Molecule annotation
1
60S Ribosomal Protein L39
Molecule annotation
1
60S Ribosomal Protein L40
Molecule annotation
1
60S Ribosomal Protein L41
Molecule annotation
1
60S Ribosomal Protein L42
Molecule annotation
1
60S Ribosomal Protein L43
Molecule annotation
1
60S Acidic Ribosomal Protein P0
Molecule annotation
1
Guanine Nucleotide-binding Protein Subunit Beta-like Protein
Molecule annotation
1
40S Ribosomal Protein S0
Molecule annotation
1
40S Ribosomal Protein S1
Molecule annotation
1
40S Ribosomal Protein S2
Molecule annotation
1
40S Ribosomal Protein S3
Molecule annotation
1
40S Ribosomal Protein S4
Molecule annotation
1
40S Ribosomal Protein S5
Molecule annotation
1
40S Ribosomal Protein S6
Molecule annotation
1
40S Ribosomal Protein S7
Molecule annotation
1
40S Ribosomal Protein S8
Molecule annotation
1
40S Ribosomal Protein S9
Molecule annotation
1
40S Ribosomal Protein S10
Molecule annotation
1
40S Ribosomal Protein S11
Molecule annotation
1
40S Ribosomal Protein S12
Molecule annotation
1
40S Ribosomal Protein S13
Molecule annotation
1
40S Ribosomal Protein S14
Molecule annotation
1
40S Ribosomal Protein S15
Molecule annotation
1
40S Ribosomal Protein S16
Molecule annotation
1
40S Ribosomal Protein S17
Molecule annotation
1
40S Ribosomal Protein S18
Molecule annotation
1
40S Ribosomal Protein S19
Molecule annotation
1
40S Ribosomal Protein S20
Molecule annotation
1
40S Ribosomal Protein S21
Molecule annotation
1
40S Ribosomal Protein S22
Molecule annotation
1
40S Ribosomal Protein S23
Molecule annotation
1
40S Ribosomal Protein S24
Molecule annotation
1
40S Ribosomal Protein S25
Molecule annotation
1
40S Ribosomal Protein S26
Molecule annotation
1
40S Ribosomal Protein S27
Molecule annotation
1
40S Ribosomal Protein S28
Molecule annotation
1
40S Ribosomal Protein S29
Molecule annotation
1
40S Ribosomal Protein S30
Molecule annotation
1
40S Ribosomal Protein S31
Molecule annotation
Nucleotides(6 molecules)
1
25S Ribosomal RNA
Molecule annotation
1
5.8s Ribosomal RNA
Molecule annotation
1
5S Ribosomal RNA
Molecule annotation
1
18S Ribosomal RNA
Molecule annotation
2
P/e-site Initiator Transfer Rnafmet
Molecule annotation
1
Messenger RNA
Molecule annotation
* Click molecule labels to explore molecular sequence information.

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