1VSP: Interactions And Dynamics Of The Shine-Dalgarno Helix In The 70s Ribosome

Citation:
Abstract
The crystal structure of an initiation-like 70S ribosome complex containing an 8-bp Shine-Dalgarno (SD) helix was determined at 3.8-A resolution. Translation-libration-screw analysis showed that the inherent anisotropic motions of the SD helix were biased along its helical axis, suggesting that during the first step of translocation, the SD helix moves along its helical screw axis. Contacts between the SD helix and the ribosome were primarily through interactions with helices 23a, 26, and 28 of 16S rRNA. Contact with the neck (helix 28) of the 30S subunit near its hinge point suggests that formation of the SD helix could affect positioning of the head of the 30S subunit for optimal interaction with initiator tRNA. The bulged U723 in helix 23a interacts with the minor groove of the SD helix at the C1539.G-10 base pair, explaining its selective conservation in bacteria and archaea.
MMDB ID: 99545
PDB Deposition Date: 2007/7/18
Updated in MMDB: 2012/07
Experimental Method:
x-ray diffraction
Resolution: 3.83  Å
Source Organism:
Similar Structures:
Merged PDB IDs:1VSP 2QNH
Asymmetric Unit for 1VSP: 54-meric
Molecular Components in 1VSP
Label Count Molecule
Proteins (48 molecules)
1
50S Ribosomal Protein L1
Molecule annotation
1
50S Ribosomal Protein L2
Molecule annotation
1
50S Ribosomal Protein L3
Molecule annotation
1
50S Ribosomal Protein L4
Molecule annotation
1
50S Ribosomal Protein L5
Molecule annotation
1
50S Ribosomal Protein L6
Molecule annotation
1
50S Ribosomal Protein L9
Molecule annotation
1
50S Ribosomal Protein L13
Molecule annotation
1
50S Ribosomal Protein L14
Molecule annotation
1
50S Ribosomal Protein L15
Molecule annotation
1
50S Ribosomal Protein L16
Molecule annotation
1
50S Ribosomal Protein L17
Molecule annotation
1
50S Ribosomal Protein L18
Molecule annotation
1
50S Ribosomal Protein L19
Molecule annotation
1
50S Ribosomal Protein L20
Molecule annotation
1
50S Ribosomal Protein L21
Molecule annotation
1
50S Ribosomal Protein L22
Molecule annotation
1
50S Ribosomal Protein L23
Molecule annotation
1
50S Ribosomal Protein L24
Molecule annotation
1
50S Ribosomal Protein L25
Molecule annotation
1
50S Ribosomal Protein L27
Molecule annotation
1
LSU Ribosomal Protein L28p
Molecule annotation
1
50S Ribosomal Protein L29
Molecule annotation
1
LSU Ribosomal Protein L30p
Molecule annotation
1
50S Ribosomal Protein L32
Molecule annotation
1
50S Ribosomal Protein L34
Molecule annotation
1
50S Ribosomal Protein L35
Molecule annotation
1
50S Ribosomal Protein L36
Molecule annotation
1
30S Ribosomal Protein S2
Molecule annotation
1
30S Ribosomal Protein S3
Molecule annotation
1
30S Ribosomal Protein S4
Molecule annotation
1
30S Ribosomal Protein S5
Molecule annotation
1
30S Ribosomal Protein S6
Molecule annotation
1
30S Ribosomal Protein S7
Molecule annotation
1
30S Ribosomal Protein S8
Molecule annotation
1
30S Ribosomal Protein S9
Molecule annotation
1
30S Ribosomal Protein S10
Molecule annotation
1
30S Ribosomal Protein S11
Molecule annotation
1
30S Ribosomal Protein S12
Molecule annotation
1
30S Ribosomal Protein S13
Molecule annotation
1
30S Ribosomal Protein S14 Type Z
Molecule annotation
1
30S Ribosomal Protein S15
Molecule annotation
1
30S Ribosomal Protein S16
Molecule annotation
1
30S Ribosomal Protein S17
Molecule annotation
1
30S Ribosomal Protein S18
Molecule annotation
1
30S Ribosomal Protein S19
Molecule annotation
1
30S Ribosomal Protein S20
Molecule annotation
1
30S Ribosomal Protein THX
Molecule annotation
Nucleotides(6 molecules)
1
23S Large Subunit Ribosomal RNA
Molecule annotation
1
5S Large Subunit Ribosomal RNA
Molecule annotation
1
16S RNA
Molecule annotation
1
P-site Trnafmet
Molecule annotation
1
E-site tRNA
Molecule annotation
1
mRNA
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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