1VJ6: PDZ2 from PTP-BL in complex with the C-terminal ligand from the APC protein

Citation:
Abstract
Understanding the basis of communication within protein domains is a major challenge in structural biology. We present structural and dynamical evidence for allosteric effects in a PDZ domain, PDZ2 from the tyrosine phosphatase PTP-BL, upon binding to a target peptide. The NMR structures of its free and peptide-bound states differ in the orientation of helix alpha2 with respect to the remainder of the molecule, concomitant with a readjustment of the hydrophobic core. Using an ultrafast mixing instrument, we detected a deviation from simple bimolecular kinetics for the association with peptide that is consistent with a rate-limiting conformational change in the protein (k(obs) approximately 7 x 10(3) s(-1)) and an induced-fit model. Furthermore, the binding kinetics of 15 mutants revealed that binding is regulated by long-range interactions, which can be correlated with the structural rearrangements resulting from peptide binding. The homologous protein PSD-95 PDZ3 did not display a similar ligand-induced conformational change.
PDB ID: 1VJ6Download
MMDB ID: 35439
PDB Deposition Date: 2004/2/3
Updated in MMDB: 2005/11
Experimental Method:
solution nmr
Source Organism:
Mus musculus
Similar Structures:
Molecular Components in 1VJ6
Label Count Molecule
Proteins (2 molecules)
1
Protein-tyrosine-phosphatase (Nonreceptor Type 13)(Gene symbol: Ptpn13)
Molecule annotation
1
Adenomatous Polyposis Coli Protein(Gene symbol: Apc)
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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