1VBK: Crystal Structure Of Ph1313 From Pyrococcus Horikoshii Ot3

Citation:
Abstract
The putative thiamine-biosynthesis protein PH1313 from Pyrococcus horikoshii OT3 has been overexpressed and purified. Crystallization was performed by the oil-microbatch method using 28%(v/v) 2-methyl-2,4-pentanediol as a precipitant at 291 K. A native X-ray diffraction data set at 1.9 A resolution and a single anomalous dispersion data set from a selenomethionine-derivative crystal at 2.1 A resolution were collected using synchrotron radiation at 100 K. The native crystal belongs to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 71.7, b = 71.2, c = 141.8 A.
PDB ID: 1VBKDownload
MMDB ID: 32770
PDB Deposition Date: 2004/2/27
Updated in MMDB: 2007/11
Experimental Method:
x-ray diffraction
Resolution: 1.9  Å
Source Organism:
Similar Structures:
Biological Unit for 1VBK: dimeric; determined by author
Molecular Components in 1VBK
Label Count Molecule
Proteins (2 molecules)
2
Hypothetical Protein Ph1313
Molecule annotation
Chemicals (4 molecules)
1
4
* Click molecule labels to explore molecular sequence information.

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