1UZB: 1-pyrroline-5-carboxylate Dehydrogenase

Citation:
Abstract
Delta(1)-pyrroline-5-carboxylate dehydrogenase (P5CDh) plays an important role in the metabolic pathway from proline to glutamate. It irreversibly catalyzes the oxidation of glutamate-gamma-semialdehyde, the product of the non-enzymatic hydrolysis of Delta(1)-pyrroline-5-carboxylate, into glutamate with the reduction of NAD(+) into NADH. We have confirmed the P5CDh activity of the Thermus thermophilus protein TT0033 (TtP5CDh), and determined the crystal structure of the enzyme in the ligand-free form at 1.4 A resolution. To investigate the structural basis of TtP5CDh function, the TtP5CDh structures with NAD(+), with NADH, and with its product glutamate were determined at 1.8 A, 1.9 A, and 1.4 A resolution, respectively. The solved structures suggest an overall view of the P5CDh catalytic mechanism and provide insights into the P5CDh deficiencies in the case of the human type II hyperprolinemia.
PDB ID: 1UZBDownload
MMDB ID: 27156
PDB Deposition Date: 2004/3/9
Updated in MMDB: 2007/10
Experimental Method:
x-ray diffraction
Resolution: 1.4  Å
Source Organism:
Similar Structures:
Biological Unit for 1UZB: hexameric; determined by author and by software (PQS)
Molecular Components in 1UZB
Label Count Molecule
Proteins (6 molecules)
6
1-pyrroline-5-carboxylate Dehydrogenase
Molecule annotation
Chemicals (18 molecules)
1
18
* Click molecule labels to explore molecular sequence information.

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