1UYT: Acetyl-Coa Carboxylase Carboxyltransferase Domain

Citation:
Abstract
Acetyl-CoA carboxylases (ACCs) are crucial for the metabolism of fatty acids, making these enzymes important targets for the development of therapeutics against obesity, diabetes, and other diseases. The carboxyltransferase (CT) domain of ACC is the site of action of commercial herbicides, such as haloxyfop, diclofop, and sethoxydim. We have determined the crystal structures at up to 2.5-A resolution of the CT domain of yeast ACC in complex with the herbicide haloxyfop or diclofop. The inhibitors are bound in the active site, at the interface of the dimer of the CT domain. Unexpectedly, inhibitor binding requires large conformational changes for several residues in this interface, which create a highly conserved hydrophobic pocket that extends deeply into the core of the dimer. Two residues that affect herbicide sensitivity are located in this binding site, and mutation of these residues disrupts the structure of the domain. Other residues in the binding site are strictly conserved among the CT domains.
PDB ID: 1UYTDownload
MMDB ID: 27149
PDB Deposition Date: 2004/3/2
Updated in MMDB: 2004/11
Experimental Method:
x-ray diffraction
Resolution: 2.5  Å
Source Organism:
Similar Structures:
Biological Unit for 1UYT: dimeric; determined by author and by software (PISA)
Molecular Components in 1UYT
Label Count Molecule
Proteins (2 molecules)
2
Acetyl-coa Carboxylase(Gene symbol: ACC1)
Molecule annotation
* Click molecule labels to explore molecular sequence information.

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