1UP6: Structure Of The 6-Phospho-Beta Glucosidase From Thermotoga Maritima At 2.55 Angstrom Resolution In The Tetragonal Form With Manganese, Nad+ And Glucose-6-Phosphate

Citation:
Abstract
Among the numerous well-characterized families of glycosidases, family 4 appears to be the anomaly, requiring both catalytic NAD+ and a divalent metal for activity. The unusual cofactor requirement prompted the proposal of a mechanism involving key NAD+-mediated redox steps as well as elimination of the glycosidic oxygen. Primary kinetic isotope effects for the 2- and 3-deutero substrate analogues, isotopic exchange with solvent, and structural analysis of a 6-phospho-beta-glucosidase, BglT (E.C. 3.2.1.6), provided evidence in support of the proposed mechanism, which has striking resemblances to that of the sugar dehydratases. Furthermore, analysis of the stereochemical outcome indicated that family 4 enzymes are retaining glycosidases.
PDB ID: 1UP6Download
MMDB ID: 29146
PDB Deposition Date: 2003/9/29
Updated in MMDB: 2012/11
Experimental Method:
x-ray diffraction
Resolution: 2.55  Å
Source Organism:
Similar Structures:
Biological Unit for 1UP6: tetrameric; determined by author and by software (PQS)
Molecular Components in 1UP6
Label Count Molecule
Proteins (4 molecules)
4
6-phospho-beta-glucosidase(Gene symbol: TM1281)
Molecule annotation
Chemicals (10 molecules)
1
2
2
4
3
2
4
2
* Click molecule labels to explore molecular sequence information.

Citing MMDB
.