1UCB: Structure Of Uncomplexed Fab Compared To Complex (1cly, 1clz)

The X-ray structure of the uncomplexed human chimeric Fab' of the anti-tumor antibody BR96 has been determined at 2.6 A resolution. The structure has been compared with Lewis Y antigen-complexed structures of BR96 which were determined previously. The comparison reveals segmental motions and/or conformational rearrangements of three CDR loops (L1, L3, and H2), whereas CDR H3 does not undergo changes upon complexation despite its significant main-chain contacts to the carbohydrate antigen. In light of the uncomplexed chimeric Fab' structure reported here, the previously observed high mobility of the CL:CH1 domains of the complexed chimeric BR96 Fab is rationalized as a "swinging" motion approximately about the axis of the elbow bend.
PDB ID: 1UCBDownload
MMDB ID: 5000
PDB Deposition Date: 1996/2/6
Updated in MMDB: 1998/11
Experimental Method:
x-ray diffraction
Resolution: 2.5  Å
Source Organism:
Similar Structures:
Biological Unit for 1UCB: dimeric; determined by author and by software (PISA)
Molecular Components in 1UCB
Label Count Molecule
Proteins (2 molecules)
Chimeric Human/mouse IGG FAB Fragment Br96(Gene symbol: IGKC)
Molecule annotation
Chimeric Human/mouse IGG FAB Fragment Br96
Molecule annotation
Chemical (1 molecule)
* Click molecule labels to explore molecular sequence information.

Citing MMDB