1U5O: Structure Of The D23a Mutant Of The Nuclear Transport Carrier Ntf2

Citation:
Abstract
Nuclear transport carriers interact with proteins of the nuclear pore complex (NPC) to transport their cargo across the nuclear envelope. One such carrier is nuclear transport factor 2 (NTF2), whose import cargo is the small GTPase Ran. A domain highly homologous to the small NTF2 protein (14kDa) is also found in a number of additional proteins, which together make up the NTF2 domain containing superfamily of proteins. Using structural, computational and biochemical analysis we have identified a functional site that is present throughout this superfamily, and our results indicate that this site functions as an NPC binding site in NTF2. Previously we showed that a D23A mutant of NTF2 exhibits increased affinity for the NPC. The mechanism of this mutation, however, was unknown as this region of NTF2 had not been implicated in binding to NPC proteins. Here we show that the D23A mutation in NTF2 does not result in gross structural changes affecting other known NPC binding sites. Instead, the D23 residue is located in an evolutionarily important region in the NTF2 domain containing superfamily, that in NTF2, is involved in binding to the NPC.
PDB ID: 1U5ODownload
MMDB ID: 30932
PDB Deposition Date: 2004/7/28
Updated in MMDB: 2007/10
Experimental Method:
x-ray diffraction
Resolution: 2.5  Å
Source Organism:
Similar Structures:
Biological Unit for 1U5O: dimeric; determined by author and by software (PISA)
Molecular Components in 1U5O
Label Count Molecule
Proteins (2 molecules)
2
Nuclear Transport Factor 2(Gene symbol: Nutf2)
Molecule annotation
* Click molecule labels to explore molecular sequence information.

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