1U3E: Dna Binding And Cleavage By The Hnh Homing Endonuclease I-Hmui

Citation:
Abstract
The structure of I-HmuI, which represents the last family of homing endonucleases without a defining crystallographic structure, has been determined in complex with its DNA target. A series of diverse protein structural domains and motifs, contacting sequential stretches of nucleotide bases, are distributed along the DNA target. I-HmuI contains an N-terminal domain with a DNA-binding surface found in the I-PpoI homing endonuclease and an associated HNH/N active site found in the bacterial colicins, and a C-terminal DNA-binding domain previously observed in the I-TevI homing endonuclease. The combination and exchange of these features between protein families indicates that the genetic mobility associated with homing endonucleases extends to the level of independent structural domains. I-HmuI provides an unambiguous structural connection between the His-Cys box endonucleases and the bacterial colicins, supporting the hypothesis that these enzymes diverged from a common ancestral nuclease.
PDB ID: 1U3EDownload
MMDB ID: 95149
PDB Deposition Date: 2004/7/21
Updated in MMDB: 2011/11
Experimental Method:
x-ray diffraction
Resolution: 2.92  Å
Source Organism:
synthetic construct
Similar Structures:
Biological Unit for 1U3E: tetrameric; determined by author
Molecular Components in 1U3E
Label Count Molecule
Protein (1 molecule)
1
HNH Homing Endonuclease(Gene symbol: 31.2)
Molecule annotation
Nucleotides(3 molecules)
1
36-mer
Molecule annotation
1
5'-d(*cp*tp*tp*ap*cp*gp*tp*gp*gp*gp*ap*ap*tp*tp*gp*cp*tp*gp *ap*gp*c)-3'
Molecule annotation
1
5'-d(p*gp*tp*tp*ap*gp*gp*cp*tp*cp*ap*tp*tp*ap*cp*t)-3'
Molecule annotation
Chemicals (13 molecules)
1
4
2
7
3
1
4
1
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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