1TRO: Crystal Structure Of Trp Repressor Operator Complex At Atomic Resolution

The crystal structure of the trp repressor/operator complex shows an extensive contact surface, including 24 direct and 6 solvent-mediated hydrogen bonds to the phosphate groups of the DNA. There are no direct hydrogen bonds or non-polar contacts to the bases that can explain the repressor's specificity for the operator sequence. Rather, the sequence seems to be recognized indirectly through its effects on the geometry of the phosphate backbone, which in turn permits the formation of a stable interface. Water-mediated polar contacts to the bases also appear to contribute part of the specificity.
PDB ID: 1TRODownload
MMDB ID: 51558
PDB Deposition Date: 1992/8/30
Updated in MMDB: 2007/10
Experimental Method:
x-ray diffraction
Resolution: 1.9  Å
Source Organism:
Escherichia coli str. K-12 substr. W3110
Similar Structures:
Biological Unit for 1TRO: tetrameric; determined by author
Molecular Components in 1TRO
Label Count Molecule
Proteins (2 molecules)
Protein (Trp Repressor)(Gene symbol: trpR)
Molecule annotation
Nucleotide(1 molecule)
DNA (5'- D(*tp*gp*tp*ap*cp*tp*ap*gp*tp*tp*ap*ap*cp*tp*ap*gp*t P*ap*c)-3')
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB