1T03: Hiv-1 Reverse Transcriptase Crosslinked To Tenofovir Terminated Template-Primer (Complex P)

Citation:
Abstract
Tenofovir, also known as PMPA, R-9-(2-(phosphonomethoxypropyl)adenine, is a nucleotide reverse transcriptase (RT) inhibitor. We have determined the crystal structures of two related complexes of HIV-1 RT with template primer and tenofovir: (i) a ternary complex at a resolution of 3.0 A of RT crosslinked to a dideoxy-terminated DNA with tenofovir-diphosphate bound as the incoming substrate; and (ii) a RT-DNA complex at a resolution of 3.1 A with tenofovir at the 3' primer terminus. The tenofovir nucleotide in the tenofovir-terminated structure seems to adopt multiple conformations. Some nucleoside reverse transcriptase inhibitors, including 3TC and AZT, have elements ('handles') that project beyond the corresponding elements on normal dNTPs (the 'substrate envelope'). HIV-1 RT resistance mechanisms to AZT and 3TC take advantage of these handles; tenofovir's structure lacks handles that could protrude through the substrate envelope to cause resistance.
PDB ID: 1T03Download
MMDB ID: 104914
PDB Deposition Date: 2004/4/7
Updated in MMDB: 2012/11
Experimental Method:
x-ray diffraction
Resolution: 3.1  Å
Source Organism:
synthetic construct
Similar Structures:
Biological Unit for 1T03: hexameric; determined by author
Molecular Components in 1T03
Label Count Molecule
Proteins (4 molecules)
1
POL Polyprotein
Molecule annotation
1
POL Polyprotein
Molecule annotation
1
Monoclonal Antibody Light Chain
Molecule annotation
1
Monoclonal Antibody Heavy Chain
Molecule annotation
Nucleotides(2 molecules)
1
Synthetic Oligonucleotide Template
Molecule annotation
1
Synthetic Oligonucleotide Primer
Molecule annotation
Chemical (1 molecule)
1
1
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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